Hello everybody this is Francisco reporting in. I'm so excited to be part of the s-stem program. During this week I was assigned to identify the bacteria culture of the sample sb132. To identify this little guy I was asked to follow the Gram staining method, for this first I needed to make a 3quadrantstreak plate. This method is used to isolate the single colonies that might be present in the mixture so it will be easier to identify them. Gram staining differentiates bacteria by the chemical and physical properties of their cell walls by detecting peptidoglycan, which is present in a thick layer in Gram-positive bacteria. So that's all for now, hopefully I will have more news about the bacteria next week.